Production of biogas in the methane fermentation process is complicated and requires optimisation, among others, with better use of biomass by bacteria. One of the applied solutions is an introduction to the process of the pre-treatment stage – disintegration, the aim of which is the fragmentation of the substrate's cellular structures before it goes to the digester. The result of the process is the increase of the raw material's susceptibility to biological degradation of the substrate, the increase of speed of the methane fermentation process and efficiency of the obtained biogas. This article presents an overview of the available methods of disintegration, and provides the results of the effectiveness of pre-treatment of biomass of the agricultural origin, conducted with the use of selected chemical, thermal and physical methods.